Data format

The data from which to make a plot must be contained within a data.frame with named columns. (I can also be in a tibble or related format as long as they convert to data.frame()) The format of the data can be of two forms, long format and wide format.

In the wide format, the general convention is “1 subject = 1 line”, that is, all the information regarding a given participant must stand on a single line. In SPSS, SAS and other statistics software, this is the standard data organization. You can convert from wide format to long format, with superbToWide() or Navarro’s wideToLong’s excellent function (Navarro, 2015). When there are multiple measures in your data, they can be used using the cbind() or crange() notations: With cbind(), you must name all the variables whereas with crange() you name a range of column with the first and last only column name of the range.

In the long format, each line contains a single measure. if there are repated measures, they are on consecutive lines, but can be assigned to a participant because there is an ID column. Then the formula must include a | to indicate that the data are nested with participants’ ID.

As an illustration, we use the following ficticious data set showing the performance of 15 participants on their motivation scores on Week 1, Week 2 and Week 3 for a program to stop smoking (long format).

#Motivation data for 15 participants over three weeks in wide format:
dta <- matrix( c(
    45, 50,  59,
    47, 58,  64,
    53, 63,  72,
    57, 64,  81,
    58, 67,  86,
    61, 70,  98,
    61, 75, 104,
    63, 79, 100,
    63, 79,  84,
    71, 81,  96,
    72, 83,  82,
    74, 84,  82,
    76, 86,  93,
    84, 90,  85,
    90, 96,  89
), ncol=3, byrow=T)

# put column names and convert to data.frame:
colnames(dta) <- c("Week 1", "Week 2", "Week 3")
dta           <- as.data.frame(dta)

The mean scores per week is illustrated below:

Figure 1. Mean scores along with 95% confidence interval per week for a program to stop smoking.

Instead of cbind(`Week 1`, `Week 2`, `Week 3`), we could have used crange(`Week 1`, `Week 3`) to refer to all the columns from Week 1 to `Week3.

Note that, by default, missing data are not handled by superb. Ideally, the cells with NA must be removed or imputed prior to perform the plot. Alternatively, you can look into the summary statistic meanNArm(). We made it on purpose to be difficult to make you plot with missing data because you should have handled these prior to making your summary plot.

Step 1: Decide of the purpose of the plot

In Figure 1 above, did you look at the result of, say, Week 1, in isolation? or did you compare it to the results obtained in the other weeks? The second perspective is actually to look at the difference between the results. if such is the case, the error bar shown on this plot are actually misleading because they are too short. The reason for that is further explored in Vignette 2.

In most experiments, one condition is compared to other conditions. In that case, we are interested in pair-wise differences between means, not by single results in isolation. Yet, the regular confidence intervals are valid only for results in isolation. Whenever you wish to compare a result to other results, to examine differences between conditions (which is, most of the time), you need to adjust the confidence interval lengths so that they remain adequate inference tools.

In superb, you obtain an adjustment to error bar length with an option for the purpose of the plot. The default purpose = "single" returns stand-alone error bars (as in Figure 1); purpose = "difference" returns error bars valid for pair-wise comparisons. The minimum specification for the data frame above would therefore be

superb(
    cbind(`Week 1`, `Week 2`, `Week 3`) ~ .,
    dta, 
    WSFactors = "Moment(3)",
    adjustments = list(purpose = "difference"),
    plotStyle="line"
    )

Figure 2. Mean scores along with difference-adjusted 95% confidence interval per week for a program to stop smoking.

The first argument is the formula describing on the left-hand side the measurements, and on the right-hand side, the betwee- subject conditions. The second argument is a data.frame, here in wide format. The data frame is dta. Because we have multiple dependent variables (collected with cbind(), the third argument describe the within-subject experimental factor(s). Here, there is a single within-subject factor (WSFactors), called Moment. In within-subject factors, it is necessary to indicate how many level the factor has (here 3).

The column named in the formula must match the column names in the data.frame. If unsure, check with

head(dta)

##   Week 1 Week 2 Week 3
## 1     45     50     59
## 2     47     58     64
## 3     53     63     72
## 4     57     64     81
## 5     58     67     86
## 6     61     70     98

Note that the measurements could be named using crange() instead of cbind(). The specification crange() is used to refer to a range of variables from the first given to the last given. Here, the formula could have been given as crange(`Week 1`, `Week 3`) ~ .

The argument in which the adjustments will be listed is called adjustments which is a list with —for the moment— an adjustment for the purpose of the plot: purpose = "difference". The standalone CI is the default (it can be obtained explicitly with purpose= "single"). These two expressions, single and difference, are from Baguley (2012).

Note that the plot obtained is a ggplot object to which additional graphic directives can be added. Figure 2 was actually obtained with these commands:

superb(
    cbind(`Week 1`, `Week 2`, `Week 3`) ~ .,
    dta, 
    WSFactors = "Moment(3)",
    statistic = "mean", errorbar = "CI",
    adjustments = list(purpose = "difference"),
    plotStyle="line"
) + 
coord_cartesian( ylim = c(50,100) ) +
ylab("Mean +- 95% CI") +
labs(title="Difference-adjusted\n95% confidence interval")+
theme_gray(base_size=10) +
scale_x_discrete(labels=c("1" = "Week 1", "2" = "Week 2", "3"="Week 3"))

The graphic directives are applied to the whole plot. The reader is referred to the package ggplot2 for more on these graphic directives.

Step 2: Decide how to handle the within-subject measures

Is is known that within-subject designs are generally more powerful at detecting differences. The implication of this is that they afford more statistical power and consequently the error bars should be shorter. The stand-alone CI are oblivious to this fact; it is however possible to inform them that you used a within-subject design.

The method to handle within-subject data comes from the observation that repeated measures tend to be correlated (e.g., Goulet & Cousineau, 2019). Informing the CI of this correlation is a process called decorrelation. To this day, there exists three methods for decorrelation:

• CM: this method, called from the two authors Cousineau and Morey (Cousineau, 2005; Morey, 2008), will decorrelate the data but each measurement may have different adjustments;
• LM: this method, the first developed by Loftus and Masson (1994), will make all the error bars have the same length;
• CA: this method, called correlation-based adjustment was proposed in Cousineau (2019). As per CM, the error bars can be different in length.

The three methods were compared in Cousineau (2019) and shown to be mathematically based on the same concepts and estimating the same precision. It is therefore a matter of personal preference which one you use.

Figure 2 above will become Figure 3 if you add a decorrelation adjustment:

superb(
    cbind(`Week 1`, `Week 2`, `Week 3`) ~ .,
    dta, 
    WSFactors = "Moment(3)",
    statistic = "mean", errorbar = "CI",
    adjustments = list(purpose = "difference", decorrelation = "CM"), #new!
    plotStyle="line",
    errorbarParams = list(width = .2)
) + 
coord_cartesian( ylim = c(50,100) ) +
ylab("Mean +- 95% CI") +
labs(title="Correlation- and Difference-adjusted\n95% confidence interval")+
theme_gray(base_size=10) +
scale_x_discrete(labels=c("1" = "Week 1", "2" = "Week 2", "3"="Week 3"))

## superb::FYI: The HyunhFeldtEpsilon measure of sphericity per group are  0.545

## superb::FYI: Some of the groups' data are not spherical. Use error bars with caution.

Unless you change the options to options(superb.feedback = 'none'), the command will issue some additional information. In the present data set, \(\varepsilon\) is 0.54 which is low. A Mauchly test of sphericity indicates rejection of sphericity, so interpret the error bars with caution. All the messages issued beginning with "FYI" are just information. Hereafter, the warnings are inhibited.

Step 3: Specify the sampling procedure and the population size

The stand-alone confidence intervals are appropriate when your sample was obtained randomly, a method formally called Simple Randomize Sampling (SRS). However, this is not the only sampling method possible. Another commonly employed sampling procedure is cluster sampling (formally Cluster Randomized Sampling, CRS). The CRS is the only one (beyond SRS) where the exact adjustment is known (Cousineau & Laurencelle, 2016) and thus SRS (no adjustment) and CRS (adjustments that tend to widen the error bars) are the only two sampling adjustments currently implemented in superb.

Other sampling methods includes Stratified Sampling, Snowball Sampling, Convenience Sampling, etc., none of which have a known impact on the precision of the measures.

Also, determine if the population size is finite or infinite. When the population under scrutiny is finite, you may have a sizeable proportion of the population in your sample, which improves precision. In this case, the error bars will be shortened.

These adjustments are implemented in superb with additional adjustments, such as

# add (ficticious) cluster membership for each participant in the column "cluster"
dta$cluster <- sort(rep(1:5, 3))

superb(
    cbind(`Week 1`, `Week 2`, `Week 3`) ~ .,
    dta, 
    WSFactors = "Moment(3)",
    adjustments = list(purpose = "difference", decorrelation = "CM",
                       samplingDesign = "CRS", popSize = 100),        #new!
    plotStyle = "line",
    clusterColumn = "cluster",  # identify the column containing cluster membership
    errorbarParams = list(width = .2)
) + 
coord_cartesian( ylim = c(50,100) ) +
ylab("Mean +- 95% CI") +
labs(title="Cluster- Correlation, and Difference-adjusted\n95% confidence interval")+
theme_gray(base_size=10) +
scale_x_discrete(labels=c("1" = "Week 1", "2" = "Week 2", "3"="Week 3"))

As you can see, the adjustments can be obtained with a single option inside the adjustments list. They are cumulative, i.e., more than one adjustment can be used, depending on the situation.